方志远 副教授

所属学院：广东工业大学生物医药学院，生物工程系

导师类别：硕士生导师

主讲课程：《基因工程》，《免疫学》

科研方向：

（1）分子诊断新技术开发：疾病诊断、食品安全及环境监测新技术和试剂研制。

（2）肿瘤微环境与肿瘤发生发展机制：通过单细胞测序及蛋白组学等手段，阐明复杂的肿瘤微环境，进而研究其对肿瘤发展、肿瘤免疫应答等反应的影响。在此基础上，筛选早期诊断生物标志物和肿瘤药物开发靶点；其次，观察不同细胞的相互作用预测药物的预后效果，并通过类器官等肿瘤模型建立药物筛选方法。

个人简述

2019年广东工业大学“青年百人计划”A层次引进人才，广州市“珠江科技新星”，近年来以第一和通讯作者在《Advanced Science》、《Biosensors and Bioelectronic》、《Chemical Communications》等杂志上发表SCI收录论文20余篇，其中影响因子大于10的3篇，授权专利10项。主持国家自然科学青年基金一项，省和市基金各一项，作为骨干成员参与国家、省部级科技项目课题3项。

教育背景：

2009.9-2012.6： 中国科学院广州生物医药与健康研究院 博士，生物化学与分子生物学

2006.9-2009.7： 暨南大学 硕士，免疫学

2002.9-2006.7： 暨南大学 学士，生物医学工程

工作经历：

2019.12-今：      广东工业大学   副教授

2017.10-2019.11： 广州医科大学附属第五医院   副研究员（广州医科大学南山学者）

2016.3-2017.9：  中科院广州生物医药与健康研究院 副研究员

2013.12-2015.12： 广州医科大学附属肿瘤医院 博士后

2012.7-2013.10：  中科院广州生物医药与健康研究院 助理研究员/副研究员

发表论文：

1) Dou Wang, Chenchen Ge, Weiyuan Liang, Qinhe Yang, Quan Liu, Wei Ma, Lulin Shi,Hong Wu, Yuhua Zhang, Zongze Wu, Chaoying Wei, Luodan Huang, Zhiyuan Fang,*Liping Liu,* Shiyun Bao,* and Han Zhang*.In Vivo Enrichment and Elimination of Circulating Tumor Cells by Using a Black Phosphorus and Antibody Functionalized Intravenous Catheter. Advanced Science, 2020,6. DOI:10.1002/advs.202000940.

2) Ning Sun, Dou Wang, Guoqiang Yao, Xiaomei Li, Ting Mei, Xinke Zhou, Kwok-Yin Wong, Baishan Jiang*, Zhiyuan Fang*. pH dependent and cathepsin B activable CaCO₃ nanoprobe for targeted in vivo tumor imaging. International Journal of Nanomedicine, 2019:14 1–9.

3) Ting mei, Xuewen Lu, Ning Sun, Xiaomei Li, Jitao Chen, Min Liang, Xinke Zhou, Zhiyuan Fang*. Real-time quantitative PCR detection of circulating tumor cells using tag DNA mediated signal amplification strategy. Journal of Pharmaceutical and Biomedical Analysis. 2018, 158 (2018) 204–208.

4) Zhiyuan Fang^#, Sichao Zheng^#, Kin-FaiChan*, Wenchang Yuana, Qi Guo, Wenyu Wu, Hoi-KuiLui, Yujing Lu, Yun-Chung Leung, Tak-Hang Chan, Kwok-Yin Wong*, NingSun*. Design, synthesis and antibacterial evaluation of 2,4-disubstituted-6-thiophenyl-pyrimidines. Eur J Med Chem. 2019,161:141-153.

5) Xuewen Lu, Ting mei, Qi Guo, Wenjing Zhou, Xiaomei Li, Jitao Chen, Xinke Zhou*, Ning Sun*, Zhiyuan Fang*. Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles. Microchimica Acta. 2018, 4;186(1):2.

6) Wei Wu*, Meng Zhou, Hong He, Chunzhao Liu, Peifeng Li, Man Wang, Ying Liu, Xiaodan Haoa, Zhiyuan Fang*. A sensitive aptasensor for the detection of Vibrio parahaemolyticus. Sensors & Actuators: B. Chemical. 2018,272 (2018) 550–558.

7) Zhiyuan Fang, Li Ban, Yanan Li, Wenchang Yuan, Zhihua Liu, Ting Liu, Xiaomei Li, Kwok-yin Wong, Yujing Lu, Ning Sun*, Xingang Yao*. A quinoline-based FtsZ inhibitor for the study of antimicrobial activity and synergistic effects with β-lactam antibiotics. J Pharmacol Sci. 2018. 137(3):283-289.

8) Wei Wu, Ning Sun, Simin Zhu, Xiaomei Li, Jitao Chen, Chunzhao Liu, Zhiyuan Fang* and Qingli Yang*. Ultrasensitive strips for the quadruple detection of nitrofuran metabolite residues. RSC Adv., 2019,9,2812-2815.

9) Ning Sun, Qi Guo, Xiaomei Li, Jitao Chen, Xiaowen Liu, Kwok-Yin Wong* and Zhiyuang Fang*. An isothermal single base extension based lateral flow biosensor and electrochemical assay for gene point mutation detection. Analytical Methods. 2018, 10, 2863-2868.

10) Wei Wu*, Lin Wang, Yang Liu, Dou Wang, Tulong Huang, Baishan Jiang and Zhiyuan Fang*. Site-specific DNA methylation detection based on enzyme-linked immunosorbent assay using recombinant methyl-CpG binding protein. Clin Chem Lab Med. 2018, 56 (7):e164.

11) Tingyu Yu, Jiao Lin, Jin Zhao, Wei Huang, Lingwen Zeng, Zhiyuan Fang ^*, Ning Xu ^*. A simple in vitro tumor chemosensitivity assay based on cell penetrating peptide tagged luciferase. Plos one. 2017,12(11):e0186184.

12) Zhiyuan Fang, Yu Wang, Hao Li, Shuaishuai Yu, Ziying Liu, Zhichao Fan, Xiaomin Chen, Yuying Wu, Xuebo Pan, Xiaokun Li, and Cong Wang. Combination treatment of citral potentiates the efficacy of hyperthermic intraperitoneal chemoperfusion with pirarubicin for colorectal cancer. Mol Pharm. 2017,14(10):3588-3597.

13) Xuewen Lu, Xiaoling Liang, Jianghong Dong, Zhiyuan Fang*, Lingwen Zeng*. Lateral flow biosensor for multiplex detection of nitrofuran metabolites based on functionalized magnetic beads. Anal Bioanal Chem. 2016 Jul 20. 408(24):6703-9.

14) Xiaoling Liang,  Lin Wang, Dou Wang,  Lingwen Zeng* and  Zhiyuan Fang*.  Portable and quantitative monitoring of mercury ions using DNA-gated mesoporous silica nanoparticles using a glucometer readout. Chemical Communications. 2016, DOI: 10.1039/C5CC08611F. 52(10):2192-4.

15) Ruan Qiang, Fang Zhiyuan, Cui Shuzhong*, Zhang Xiangliang, Wu Yinbing, Tang Hongsheng, Tu Yinuo, Ding Yan. Thermo-chemotherapy Induced miR-218 upregulation inhibits the invasion of gastric cancer via targeting Gli2 and E-cadherin. Tumor Biol. 2015, 36(8):5807-14.

16) Wei Wu^#, Zhiyuan Fang^#, Shiming Zhao, Xuewen Lu, Luxin Yu, Ting Mei, Lingwen Zeng*. A simple aptamer biosensor for Salmonellae enteritidis based on fluorescence-switch signaling graphene oxide. RSC Advances, 2014, 4, 42, 22009.

17) Huang Baotian^#, Fang Zhiyuan^#, Huang Y, Lin P, Chen Z*. A dosimetric analysis of volumetric-modulated arc radiotherapy with jaw width restriction vs 7 field intensity-modulated radiotherapy for definitive treatment of cervical cancer. The British journal of radiology. 2014, 87(1039):20140183.

18) Zhiyuan Fang, Wei Wu, Xuewen Lu, Lingwen Zeng*. Lateral flow biosensor for DNA extraction-free detection of salmonella based on aptamer mediated strand displacement amplification. Biosensors and Bioelectronics. 2014, 15(56) 192-197.

19) Zhiyuan Fang, Baishan Jiang, Wei Wu, Zhicheng Xiang, Chuanyan Ouyang, tulong Huang, Junhua Chen and Lingwen Zeng*. ELISA detection of semicarbazide based on a fast sample pretreatment method. Chemical Communications. 2013, 49(55):6164-6.

20) Zhiyuan Fang, Wenjuan Zhang, Chenchen Ge, Jie Liu, Puchang Lie, and Lingwen Zeng*. A sandwich assay for quantitative detection of transcription factor in cell lysate. Analyst. 2012, 137 (18), 4127 - 4130.

21) Zhiyuan Fang, Chenchen Ge, Wenjuan Zhang, Puchang Lie, Lingwen Zeng*. A lateral flow biosensor for rapid detection of DNA-binding protein c-jun. Biosensors and Bioelectronics.  2011, (27) 192-196.

22) Zhiyuan Fang, Jing Huang, Puchang Lie, Zhuo Xiao, Chuanyan Ouyang, Qing Wu, Yixing Wu, Guodong Liu and Lingwen Zeng*. Lateral flow nucleic acid biosensor for Cu²⁺ detection in aqueous solution with high sensitivity and selectivity. Chemical Communications. 2010, 46, 9043-9045.

23) Zhiyuan Fang, Feiyue Xing*, Bronner C, et al. ICBP90 mediates the ERK1/2 signaling to regulate the proliferation of Jurkat T cells. Cellular Immunology. 2009; 257(1-2):80-7.

24) 方志远; 邢飞跃*; 郭中锋; 陈玲. Jagged-1对骨髓来源树突状细胞生成细胞因子的影响。细胞与分子免疫学杂志，2009-02-18。

25) 方志远; 邢飞跃*. Jagged 1促进肿瘤发生。生物学杂志，2008-12-18。

26) 方志远; 邢飞跃*. Jagged-1在肿瘤新生血管形成中的双重作用。中国肿瘤生物治疗杂志，2008-04-30。

27) Junhua Chen, Zhiyuan Fang, Puchang Lie, and Lingwen Zeng*. Computational lateral flow biosensor for proteins and small molecules: A new class of strip logic gates. Anal. Chem. 2012, 84, 6321-6325.

28) Chenchen Ge, Zhiyuan Fang, Junhua Chen, Jie Liu, Xuewen Lu and Lingwen Zeng*. A simple colorimetric detection of DNA methylation. Analyst. 2012, 137, 2032-2035.

29) Junhua Chen, Zhiyuan Fang, Jie Liu, Lingwen Zeng*. A Simple and rapid biosensor for ochratoxin A based on a structure-switching signaling sptamer. Food Control. 2012,25, 555-560.

30) Wu W, Yu L, Fang Z, Lie P, Zeng L*. A lateral flow biosensor for the detection of human pluripotent stem cells. Anal Biochem. 2013 Feb 12. doi:pii: S0003-2697(13)00063-8. 10.1016/j.ab.2013.01.034.

31) Liu J, Chen J, Fang Z, Zeng L*. A simple and sensitive sensor for rapid detection of sulfide anions using DNA-templated copper nanoparticles as fluorescent probes. Analyst. 2012 Dec 7;137(23):5502-5. doi: 10.1039/c2an35885a.

32) Xiao Z, Lie P, Fang Z, Yu L, Chen J, Liu J, Ge C, Zhou X, Zeng L*. A lateral flow biosensor for detection of single nucleotide polymorphism by circular strand displacement reaction. Chem Commun. 2012 Sep 4;48(68):8547-9. doi: 10.1039/c2cc33931e.

33) Chen J, Liu J, Fang Z, Zeng L*. Random dsDNA-templated formation of copper nanoparticles as novel fluorescence probes for label-free lead ions detection. Chem Commun. 2012 Jan 25;48(7):1057-9. doi: 10.1039/c2cc16668b.

34) Lie P, Liu J, Fang Z, Dun B, Zeng L*. A lateral flow biosensor for detection of nucleic acids with high sensitivity and selectivity. Chem Commun. 2012 Jan 7;48(2):236-8. doi: 10.1039/c1cc15878c.

35) Wu W, Chen J, Fang Z, Ge C, Xiang Z, Ouyang C, Lie P, Xiao Z, Yu L, Wang L, Zeng L*. A self-assembled deoxyribonucleic acid concatemer for sensitive detection of single nucleotide polymorphism. Anal Chim Acta. 2013 Dec 4; 804:235-9. doi: 10.1016/j.aca.2013.09.055.

36) Yu L, Wu W, Chen J, Xiao Z, Ge C, Lie P, Fang Z, Chen L, Zhang Y, Zeng L*. Spectrophotometric and ultrasensitive DNA bioassay by circular-strand displacement polymerization reaction. Analyst. 2013 Dec 7;138(23):7182-7. doi: 10.1039/c3an01347b. Epub 2013 Oct 15.

37) Ge C, Yu L, Fang Z, Zeng L*. An enhanced strip biosensor for rapid and sensitive detection of histone methylation. Anal Chem. 2013 Oct 1;85(19):9343-9. doi: 10.1021/ac402202x. Epub 2013 Sep 11.

38) Ge C, Chen J, Wu W, Fang Z, Chen L, Liu Q, Wang L, Xing X, Zeng L*. An enzyme-free and label-free assay for copper(II) ion detection based on self-assembled DNA concatamers and Sybr Green I. Analyst. 2013 Sep 7;138(17):4737-40. doi: 10.1039/c3an00973d. Epub 2013 Jul .

39) Wu W, Zhao S, Mao Y, Fang Z, Lu X, Zeng L*. A sensitive lateral flow biosensor for Escherichia coli O157:H7 detection based on aptamer mediated strand displacement amplification. Anal Chim Acta. 2015 Feb 25; 861:62-8.

40) Zhang XL, Shi HJ, Wang JP, Tang HS, Wu YB, Fang ZY, Cui SZ, Wang LT*. MicroRNA-218 is upregulated in gastric cancer after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy and increases chemosensitivity to cisplatin. World J Gastroenterol. 2014 Aug 28;20(32):11347-55.

主持（参与）承担的主要项目（基金、工程）情况

授权专利情况